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OBJECTIVE:To study the chemical components of Swertia nervosa. METHODS:Silica gel column chromatogra-phy was used for purification and analysis of compounds’structure based on physicochemical properties and spectral data. RE-SULTS:Five compounds were isolated and identified in petroleum ether portion of S. nervosa,involving 1-hydroxy-3,7,8-trime-thoxyxanthone (1),1,8-dihydroxy-3,7-dimethoxyxanthone (2),1,8-dihydroxy-3,5-dimethoxyxanthone(3),1-hydroxy-3,5-dime-thoxyxanthone(4)and β-sitosterol(5). CONCLUSIONS:Compound 1,3 and 4 are isolated from S. nervosa for the first time,and the study has laid a foundation for the quality evaluation of S. nervosa.
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OBJECTIVE To study the distribution and antimicrobial resistance of Acinetobacter baumannii infection in our hospital.METHODS Five hundred and sixty-nine strains of A.baumannii isolated from patients with infection from Jan 2001 to Dec 2005 were collected and antimicrobial susceptibility tests were performed.RESULTS Two hundred and seventy-five strains(48.3%) of A.baumannii were from intensive care unit(ICU).Four hundred and five strains(71.2%) of A.baumannii were examined from sputum.A.baumannii had various drug resistances to 12 antibiotics,which were monitored and proved tending to strengthen.The resistance rate in the ICU was distinctly higher than the others with significant difference(P
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Objective To study diagnostic methods for early renal injury in hypertension patients. Methods Urinary microalbumin (mALB) and ? 2 microglodulin(? 2 MG) levels were measured with rate nepherometry. Total quantitative enzyme immunoassay was employed to measure urinary retinol binding protein (RBP) levels, rate for urinary N acetyl beta D glucosaminidase (NAG), and Jaffes rate for urinary creatinine (Cr). Results The levels of urinary RBP, mALB, ? 2 MG, NAG in hypertension patients were significantly higher than those in controls ( P
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Objective To study CEAmRNA、CK19mRNA and CK20mRNA in peripheral blood of lung cancer patients by Real-time RT-PCR,consequently discussing those result′s clinical significance.Methods 48 lung cancer patients,8 benign lung disease patients and 30 healthy volunteers of CEAmRNA,CK19mRNA and CK20mRNA in peripheral blood were detectd by Real-time RT-PCR.Results The levels of 48 lung cancer patients CEAmRNA in peripheral blood reach (16 264?28 765) copies/ml,of which 26 patients,results are positive; The levels of 48 lung cancer patients CK19mRNA in peripheral blood reach (14 891?27 244) copies/ml,of which 27 patients,results are positive; The levels of 48 lung cancer patients CK20mRNA in peripheral blood reach (10 924?21 678) copies/ml,of which 20 patients,results are positive. Among of 8 benign lung disease patients,there is just CK20mRNA was detected in one patient,both CEAmRNA and CK19mRNA were not detected in their peripheral blood. As for those 30 healthy volunteers,there is none of the mRNA was detected. Moreover,there is a marked positive correlation between the levels of CEAmRNA,CK19mRNA and CK20mRNA,the levels of CEAmRNA,CK19mRNA and CK20mRNA were correlated to the physiological cause or degree of lung cancer.Conclusion CEAmRNA,CK19mRNA and CK20mRNA in peripheral blood can be as auxiliary index for diagnosing lung cancer micrometastasis,also can monitor the lung cancer micrometastasis by quantity it.
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Objective:To study the clinical value of lectin reactive alpha fetoprotein(AFP) detection in primary hepatocellular carcinoma (PHC) and benign liver diseases.Methods:According to the different affinity with AFP, AFP L 3 was detected by affinity immunoelectrophoresis blotting method, AFP detected by Chemiluminescent Immunoassay.Results:Taking AFP L 3≥15% as the diagnosis criteria of PHC,the sensitivity was 91.1%,the specificity in differential diagnosis in chronical liver diseases was 95.0%,the levels of AFP L 3 has no correlation to serum AFP levels and the sizes of PHC.Conclusion:It is of great clinical value to detect AFP L 3 and serum AFP levels for PHC in early diagnosis and benign differential diagnosis in liver diseases. [
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Objective:To establish a method which could quantify human IL-8 mRNA in peripheral blood monocytes.Methods:Two probe were designed aiming at amplicon of RT-PCR,of which one being capture probe adorned 5,end by ammonia and one being monitor probe adorned 3,end by bintony.So as,the capture probe could couple with the N-oxysuccinmide esters(NOS) group on the DNA-binding wells through covalent attachment.In this way,oligonucleotides were immobilized on the plate surface and arise straightly to capture target amplicon.Total RNA was extracted from peripheral blood mononuclear cell and IL-8 mRNA was amplified by using RT-PCR.Heating denatured products and denatured probe,were then added to wells and hybridization occurs between capture probe,target amplicon and detection probe.After the addition of Avidiu-peroxidase developing system,the optical density were read at 450 nm.Results:Sensitivity,detecting PCR product of 16 PCR cycling,of 5?10~3 PBMCs and of a dilute 1∶256 were its prior.Meanwhile,this method could get a specify result and a precise of 5.2%.Conclusion:Resulting in simplicity,sensitivity and specificity results,this method may be a good choice for quantify of PCR products.